MPP5 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MPP5.
Immunogen
A synthetic peptide corresponding to human MPP5.
Sequence
NKASPPFPLISNAQ-C
Host
Goat
Theoretical MW (kDa)
77.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MPP5 polyclonal antibody (Cat # PAB6551) (1 ug/mL) staining of human brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MPP5
Entrez GeneID
64398Protein Accession#
NP_071919Gene Name
MPP5
Gene Alias
FLJ12615, PALS1
Gene Description
membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
Omim ID
606958Gene Ontology
HyperlinkGene Summary
Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001 [PubMed 11311936]). MPP5 is a member of the p55-like MAGUK subfamily.[supplied by OMIM
Other Designations
MAGUK p55 subfamily member 5|membrane protein, palmitoylated 5|stardust
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Interactome
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Pathway
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Publication Reference
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Molecular cloning and characterization of Pals, proteins associated with mLin-7.
Kamberov E, Makarova O, Roh M, Liu A, Karnak D, Straight S, Margolis B.
The Journal of Biological Chemistry 2000 Apr; 275(15):11425.
Application:IP, WB-Ce, Human, Mouse, 293 cells, Brain.
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Molecular cloning and characterization of Pals, proteins associated with mLin-7.
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