VPS45 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
VPS45 polyclonal antibody (Cat # PAB6508) staining (0.3 ug/mL) of human PBMCs lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of VPS45.
Immunogen
A synthetic peptide corresponding to human VPS45.
Sequence
C-ESSQVTSRSASRR
Host
Goat
Theoretical MW (kDa)
65
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
VPS45 polyclonal antibody (Cat # PAB6508) staining (0.3 ug/mL) of human PBMCs lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — VPS45
Entrez GeneID
11311Protein Accession#
NP_009190.2Gene Name
VPS45
Gene Alias
H1, H1VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A
Gene Description
vacuolar protein sorting 45 homolog (S. cerevisiae)
Omim ID
610035Gene Ontology
HyperlinkGene Summary
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene is a member of the Sec1 domain family, and shows a high degree of sequence similarity to mouse, rat and yeast Vps45. The exact function of this gene is not known, but its high expression in peripheral blood mononuclear cells suggests a role in trafficking proteins, including inflammatory mediators. [provided by RefSeq
Other Designations
OTTHUMP00000014482|leucocyte vacuolar protein sorting 45|vacuolar protein sorting 45A
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Interactome
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Pathway
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Publication Reference
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Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45).
Rajasekariah P, Eyre HJ, Stanley KK, Walls RS, Sutherland GR.
The International Journal of Biochemistry & Cell Biology 1999 Jun; 31(6):683.
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Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (h1Vps45).
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