UBE2K polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
UBE2K polyclonal antibody (Cat # PAB6301) staining (1.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
UBE2K polyclonal antibody (Cat # PAB6301)(3.8 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of UBE2K.
Immunogen
A synthetic peptide corresponding to C-terminus of human UBE2K.
Sequence
C-SWDVETATELLLSN
Host
Goat
Theoretical MW (kDa)
22.4
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
UBE2K polyclonal antibody (Cat # PAB6301) staining (1.5 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
UBE2K polyclonal antibody (Cat # PAB6301)(3.8 ug/mL) staining of paraffin embedded human cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBE2K
Entrez GeneID
3093Protein Accession#
NP_005330.1;NP_001104582.1;NP_001104583.1Gene Name
UBE2K
Gene Alias
DKFZp564C1216, DKFZp686J24237, E2-25K, HIP2, HYPG, LIG
Gene Description
ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
Omim ID
602846Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq
Other Designations
huntingtin interacting protein 2|ubiquitin carrier protein|ubiquitin-conjugating enzyme E2-25 KDA|ubiquitin-conjugating enzyme E2-25K|ubiquitin-protein ligase
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Interactome
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Pathway
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Publication Reference
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Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.
Kalchman MA, Graham RK, Xia G, Koide HB, Hodgson JG, Graham KC, Goldberg YP, Gietz RD, Pickart CM, Hayden MR.
The Journal of Biological Chemistry 1996 Aug; 271(32):19385.
Application：IP, WB-Ti, WB-Tr, Human, Rat, Brains, Kidneys, Livers, Lungs, Hearts, HEK 293 cells, Skeletal muscles, Spleens, Testes.
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Huntingtin is ubiquitinated and interacts with a specific ubiquitin-conjugating enzyme.
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