ING2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
ING2 polyclonal antibody (Cat # PAB6177) staining (2 ug/mL) of HepG2 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ING2.
Immunogen
A synthetic peptide corresponding to human ING2.
Sequence
C-DKSTEKTKKDRRSR
Host
Goat
Theoretical MW (kDa)
32.8
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:1000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ING2 polyclonal antibody (Cat # PAB6177) staining (2 ug/mL) of HepG2 lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ING2
Entrez GeneID
3622Protein Accession#
NP_001555Gene Name
ING2
Gene Alias
ING1L, p33ING2
Gene Description
inhibitor of growth family, member 2
Omim ID
604215Gene Ontology
HyperlinkGene Summary
This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. [provided by RefSeq
Other Designations
inhibitor of growth 1-like|inhibitor of growth family, member 1-like
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Interactome
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Publication Reference
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ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation.
Pedeux R, Sengupta S, Shen JC, Demidov ON, Saito S, Onogi H, Kumamoto K, Wincovitch S, Garfield SH, McMenamin M, Nagashima M, Grossman SR, Appella E, Harris CC.
Molecular and Cellular Biology 2005 Aug; 25(15):6639.
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ING2 regulates the onset of replicative senescence by induction of p300-dependent p53 acetylation.
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