TSPAN32 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
TSPAN32 polyclonal antibody (Cat # PAB6166) staining (2 ug/mL) of HeLa lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TSPAN32.
Immunogen
A synthetic peptide corresponding to human TSPAN32.
Sequence
GGLSGCPERGLSD
Host
Goat
Theoretical MW (kDa)
34.6
Reactivity
Human
Specificity
This antibody is expected to recognize human isoforms 1 and 2 (NP_620591.1, NP_005696.1) but not human isoforms 3 and 4 (NP_620592, NP_620593).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
TSPAN32 polyclonal antibody (Cat # PAB6166) staining (2 ug/mL) of HeLa lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — TSPAN32
Entrez GeneID
10077Protein Accession#
NP_620591.1;NP_005696.1Gene Name
TSPAN32
Gene Alias
FLJ17158, FLJ97586, MGC22455, PHEMX, PHMX, TSSC6
Gene Description
tetraspanin 32
Omim ID
603853Gene Ontology
HyperlinkGene Summary
This gene, which is a member of the tetraspanin superfamily, is one of several tumor-suppressing subtransferable fragments located in the imprinted gene domain of chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. This gene is located among several imprinted genes; however, this gene, as well as the tumor-suppressing subchromosomal transferable fragment 4, escapes imprinting. This gene may play a role in malignancies and diseases that involve this region, and it is also involved in hematopoietic cell function. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
Other Designations
pan-hematopoietic expression protein|tumor-suppressing STF cDNA 6|tumor-suppressing subchromosomal transferable fragment cDNA 6|tumor-suppressing subtransferable candidate 6
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Interactome
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Publication Reference
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Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting.
Lee MP, Brandenburg S, Landes GM, Adams M, Miller G, Feinberg AP.
Human Molecular Genetics 1999 Apr; 8(4):683.
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Two novel genes in the center of the 11p15 imprinted domain escape genomic imprinting.
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