PLK4 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
PLK4 polyclonal antibody (Cat # PAB6122) (2 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of PLK4.
Immunogen
A synthetic peptide corresponding to human PLK4.
Sequence
ATCIGEKIEDFKVGN
Host
Goat
Theoretical MW (kDa)
109
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
PLK4 polyclonal antibody (Cat # PAB6122) (2 ug/mL) staining of human colon lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — PLK4
Entrez GeneID
10733Protein Accession#
NP_055079.2Gene Name
PLK4
Gene Alias
SAK, STK18
Gene Description
polo-like kinase 4 (Drosophila)
Omim ID
605031Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. [provided by RefSeq
Other Designations
Snk akin kinase|polo-like kinase 4|serine/threonine kinase 18|serine/threonine protein kinase SAK
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Interactome
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Disease
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Publication Reference
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Transcriptional silencing of Polo-like kinase 2 (SNK/PLK2) is a frequent event in B-cell malignancies.
Nelofer Syed, Paul Smith, Alexandra Sullivan, Lindsay C Spender, Martin Dyer, Lorraine Karran, Jenny O'Nions, Martin Allday, Ingrid Hoffmann, Dorothy Crawford, Beverley Griffin, Paul J Farrell, Tim Crook.
Blood 2006 Jan; 107(1):250.
Application：WB-Ce, Human, Akata, BJAB, BL2, BL30, BL37, BL41, CR, DG75, DOHHC1, IB4, JAC, JUM2P, LK6, Namalwa, Odiambo, PD LCL, Ramos cells.
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Transcriptional silencing of Polo-like kinase 2 (SNK/PLK2) is a frequent event in B-cell malignancies.
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