ATF7 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
ATF7 polyclonal antibody (Cat # PAB6078) staining (0.5 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ATF7 polyclonal antibody (Cat # PAB6078, 3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ATF7.
Immunogen
A synthetic peptide corresponding to human ATF7.
Sequence
C-HVIMTPQSQSAGR
Host
Goat
Theoretical MW (kDa)
53.0, 51.8, 49.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (0.5-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ATF7 polyclonal antibody (Cat # PAB6078) staining (0.5 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ATF7 polyclonal antibody (Cat # PAB6078, 3.8 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATF7
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Interactome
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Publication Reference
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ATF-7, a novel bZIP protein, interacts with the PRL-1 protein-tyrosine phosphatase.
Peters CS, Liang X, Li S, Kannan S, Peng Y, Taub R, Diamond RH.
The Journal of Biological Chemistry 2001 Apr; 276(17):13718.
Application：GSA, Recombinant protein.
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ATF-7, a novel bZIP protein, interacts with the PRL-1 protein-tyrosine phosphatase.
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