ABTB1 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ABTB1.
Immunogen
A synthetic peptide corresponding to human ABTB1.
Sequence
RALEDLLVSIGLDC
Host
Goat
Theoretical MW (kDa)
37.6, 54.0, 51.1
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ABTB1 polyclonal antibody (Cat # PAB6064) staining (1 ug/mL) of human heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ABTB1 polyclonal antibody (Cat # PAB6064, 3.8 ug/mL) staining of paraffin embedded human heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ABTB1
Entrez GeneID
80325Protein Accession#
NP_115937Gene Name
ABTB1
Gene Alias
BPOZ, Btb3, EF1ABP, MGC20585, PP2259
Gene Description
ankyrin repeat and BTB (POZ) domain containing 1
Omim ID
608308Gene Ontology
HyperlinkGene Summary
This gene encodes a protein with an ankyrin repeat region and two BTB/POZ domains, which are thought to be involved in protein-protein interactions. Expression of this gene is activated by the phosphatase and tensin homolog, a tumor suppressor. Alternate splicing results in three transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
elongation factor 1A binding protein
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Interactome
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Publication Reference
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Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway.
Unoki M, Nakamura Y.
Oncogene 2001 Jul; 20(33):4457.
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Growth-suppressive effects of BPOZ and EGR2, two genes involved in the PTEN signaling pathway.
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