GRAP2 polyclonal antibody
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More Files
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Western Blot (Cell lysate)
GRAP2 polyclonal antibody (Cat # PAB6036) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Western Blot (Transfected lysate)
HEK293 overexpressing GRAP2 and probed with GRAP2 polyclonal antibody (Cat # PAB6036) (mock transfection in first lane), tested by Origene.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GRAP2.
Immunogen
A synthetic peptide corresponding to human GRAP2.
Sequence
C-GLFPANYVAPMTR
Host
Goat
Theoretical MW (kDa)
37.9
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
GRAP2 polyclonal antibody (Cat # PAB6036) staining (0.5 ug/mL) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Western Blot (Transfected lysate)
HEK293 overexpressing GRAP2 and probed with GRAP2 polyclonal antibody (Cat # PAB6036) (mock transfection in first lane), tested by Origene.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GRAP2
Entrez GeneID
9402Protein Accession#
NP_004801.1Gene Name
GRAP2
Gene Alias
GADS, GRAP-2, GRB2L, GRBLG, GRID, GRPL, GrbX, Grf40, Mona, P38
Gene Description
GRB2-related adaptor protein 2
Omim ID
604518Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq
Other Designations
GRB-2-like protein|GRB2-related protein with insert domain|OTTHUMP00000028837|SH3-SH2-SH3 adaptor molecule|growth factor receptor-binding protein|growth factor receptor-bound protein 2-related adaptor protein 2|hematopoietic cell-associated adaptor protei
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Interactome
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Pathway
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Disease
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Publication Reference
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Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1.
Qiu M, Hua S, Agrawal M, Li G, Cai J, Chan E, Zhou H, Luo Y, Liu M.
Biochemical and Biophysical Research Communications 1998 Dec; 253(2):443.
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Molecular cloning and expression of human grap-2, a novel leukocyte-specific SH2- and SH3-containing adaptor-like protein that binds to gab-1.
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