GJA1 (phospho S367) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extract from HeLa (Lane 1, 4), K-562 (Lane 2, 5) and 293 (Lane 3, 6) cells, untreated or treated with PMA (1 uM, 30 min). Using GJA1 (phospho S367) polyclonal antibody (Cat # PAB5681).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GJA1 (phospho S367) polyclonal antibody (Cat # PAB5681).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of GJA1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S367 of human GJA1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extract from HeLa (Lane 1, 4), K-562 (Lane 2, 5) and 293 (Lane 3, 6) cells, untreated or treated with PMA (1 uM, 30 min). Using GJA1 (phospho S367) polyclonal antibody (Cat # PAB5681).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GJA1 (phospho S367) polyclonal antibody (Cat # PAB5681). -
Gene Info — GJA1
Entrez GeneID
2697Gene Name
GJA1
Gene Alias
CX43, DFNB38, GJAL, ODDD
Gene Description
gap junction protein, alpha 1, 43kDa
Gene Ontology
HyperlinkGene Summary
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia and heart malformations. [provided by RefSeq
Other Designations
OTTHUMP00000017111|connexin 43|gap junction 43 kDa heart protein|gap junction protein, alpha-like
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Interactome
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Pathway
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Disease
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Publication Reference
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Alterations of phosphorylation state of connexin 43 during hypoxia and reoxygenation are associated with cardiac function.
Matsushita S, Kurihara H, Watanabe M, Okada T, Sakai T, Amano A.
The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society 2005 Nov; 54(3):343.
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Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation.
Bao X, Altenberg GA, Reuss L.
American Journal of Physiology. Cell Physiology 2004 Mar; 286(3):C647.
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Connexin43 phosphorylation at S368 is acute during S and G2/M and in response to protein kinase C activation.
Solan JL, Fry MD, TenBroek EM, Lampe PD.
Journal of Cell Science 2003 Apr; 116(Pt 11):2203.
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Alterations of phosphorylation state of connexin 43 during hypoxia and reoxygenation are associated with cardiac function.
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