YBX1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of YBX1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human YBX1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of YBX1 (arrow) using rabbit YBX1 polyclonal antibody (Cat # PAB4883). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the YBX1 gene (Lane 2) (Origene Technologies).Enzyme-linked Immunoabsorbent Assay
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Gene Info — YBX1
Entrez GeneID
4904Protein Accession#
NP_004550;P67809Gene Name
YBX1
Gene Alias
BP-8, CSDA2, CSDB, DBPB, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP-1, NSEP1, YB-1, YB1
Gene Description
Y box binding protein 1
Omim ID
154030Gene Ontology
HyperlinkGene Summary
class II
Other Designations
DNA-binding protein B|OTTHUMP00000008731|major histocompatibility complex, class II, Y box-binding protein I|nuclease sensitive element binding protein 1
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Interactome
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Publication Reference
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Expression of HER2 and estrogen receptor alpha depends upon nuclear localization of Y-box binding protein-1 in human breast cancers.
Fujii T, Kawahara A, Basaki Y, Hattori S, Nakashima K, Nakano K, Shirouzu K, Kohno K, Yanagawa T, Yamana H, Nishio K, Ono M, Kuwano M, Kage M.
Cancer Research 2008 Mar; 68(5):1504.
Application:IHC-P, WB, Human, MDA-MB-231, T-47D cells, Human breast cancer.
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Twist promotes tumor cell growth through YB-1 expression.
Shiota M, Izumi H, Onitsuka T, Miyamoto N, Kashiwagi E, Kidani A, Yokomizo A, Naito S, Kohno K.
Cancer Research 2008 Jan; 68(1):98.
Application:WB-Tr, Human, Human dermal fibroblast cells, PC-3 cells.
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Y-box protein-1 controls transforming growth factor-beta1 translation in proximal tubular cells.
Fraser DJ, Phillips AO, Zhang X, van Roeyen CR, Muehlenberg P, En-Nia A, Mertens PR.
Kidney International 2008 Mar; 73(6):724.
Application:Func, Human, HK-2 cells.
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Expression of HER2 and estrogen receptor alpha depends upon nuclear localization of Y-box binding protein-1 in human breast cancers.
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