MVP polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The MVP polyclonal antibody (Cat # PAB4800) is used in Western blot to detect MVP in A-375 cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of MVP (arrow) using rabbit MVP polyclonal antibody (Cat # PAB4800). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MVP gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinomareacted with MVP polyclonal antibody (Cat # PAB4800), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using MVP polyclonal antibody (Cat # PAB4800)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MVP.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MVP.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The MVP polyclonal antibody (Cat # PAB4800) is used in Western blot to detect MVP in A-375 cell lysate.Western Blot (Transfected lysate)
Western blot analysis of MVP (arrow) using rabbit MVP polyclonal antibody (Cat # PAB4800). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MVP gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human lung carcinomareacted with MVP polyclonal antibody (Cat # PAB4800), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using MVP polyclonal antibody (Cat # PAB4800)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — MVP
Entrez GeneID
9961Protein Accession#
NP_005106Gene Name
MVP
Gene Alias
LRP, VAULT1
Gene Description
major vault protein
Omim ID
605088Gene Ontology
HyperlinkGene Summary
This gene encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts. [provided by RefSeq
Other Designations
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Interactome
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Publication Reference
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Overexpression of the human major vault protein in gangliogliomas.
Aronica E, Gorter JA, van Vliet EA, Spliet WG, van Veelen CW, van Rijen PC, Leenstra S, Ramkema MD, Scheffer GL, Scheper RJ, Sisodiya SM, Troost D.
Epilepsia 2003 Sep; 44(9):1166.
Application：ICC, IF, IHC-P, WB, Human, Human gangliogliomas.
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RNA expression of breast cancer resistance protein, lung resistance-related protein, multidrug resistance-associated proteins 1 and 2, and multidrug resistance gene 1 in breast cancer: correlation with chemotherapeutic response.
Burger H, Foekens JA, Look MP, Meijer-van Gelder ME, Klijn JG, Wiemer EA, Stoter G, Nooter K.
Clinical Cancer Research 2003 Feb; 9(2):827.
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Expression of multidrug resistance-associated proteins in rhabdomyosarcomas before and after chemotherapy: the relationship between lung resistance-related protein (LRP) and differentiation.
Klunder JW, Komdeur R, Van Der Graaf WT, De Bont EJ, Hoekstra HJ, Van Den Berg E, Molenaar WM.
Human Pathology 2003 Feb; 34(2):150.
Application：IHC-P, Human, Human rhabdomyosarcomas.
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Overexpression of the human major vault protein in gangliogliomas.
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