MMP15 polyclonal antibody
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More Files
- More Functions
Western Blot
The MMP15 polyclonal antibody (Cat # PAB4783) is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL-60 cell lysate (lane 2) .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinomareacted with MMP15 polyclonal antibody (Cat # PAB4783), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow Cytometry
Flow cytometric analysis of WiDr cells using MMP15 polyclonal antibody (Cat # PAB4783)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MMP15.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MMP15.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
The MMP15 polyclonal antibody (Cat # PAB4783) is used in Western blot to detect MMP15 in mouse brain tissue lysate (lane 1) and HL-60 cell lysate (lane 2) .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human colon carcinomareacted with MMP15 polyclonal antibody (Cat # PAB4783), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of WiDr cells using MMP15 polyclonal antibody (Cat # PAB4783)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — MMP15
Entrez GeneID
4324Protein Accession#
MM15_HUMANGene Name
MMP15
Gene Alias
MT2-MMP, MTMMP2, SMCP-2
Gene Description
matrix metallopeptidase 15 (membrane-inserted)
Omim ID
602261Gene Ontology
HyperlinkGene Summary
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. [provided by RefSeq
Other Designations
matrix metalloproteinase 15|matrix metalloproteinase 15 (membrane-inserted)|membrane-type matrix metalloproteinase 2|membrane-type-2 matrix metalloproteinase
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Interactome
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Disease
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Publication Reference
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mRNA expression of the five membrane-type matrix metalloproteinases MT1-MT5 in human prostatic cell lines and their down-regulation in human malignant prostatic tissue.
Jung M, Romer A, Keyszer G, Lein M, Kristiansen G, Schnorr D, Loening SA, Jung K.
Prostate 2003 May; 55(2):89.
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Matrix metalloproteinases.
Nagase H, Woessner JF Jr.
The Journal of Biological Chemistry 1999 Jul; 274(31):21491.
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Identification of soluble type of membrane-type matrix metalloproteinase-3 formed by alternatively spliced mRNA.
Matsumoto S, Katoh M, Saito S, Watanabe T, Masuho Y.
Biochimica et Biophysica Acta 1997 Nov; 1354(2):159.
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mRNA expression of the five membrane-type matrix metalloproteinases MT1-MT5 in human prostatic cell lines and their down-regulation in human malignant prostatic tissue.
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