MAGEA9 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
The MAGEA9 polyclonal antibody (Cat # PAB4750) is used in Western blot to detect MAGEA9 in placenta tissue lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAGEA9 polyclonal antibody (Cat # PAB4750) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAGEA9.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human MAGEA9.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
The MAGEA9 polyclonal antibody (Cat # PAB4750) is used in Western blot to detect MAGEA9 in placenta tissue lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MAGEA9 polyclonal antibody (Cat # PAB4750) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAGEA9
Entrez GeneID
4108Protein Accession#
NP_005356Gene Name
MAGEA9
Gene Alias
MAGE9, MGC8421
Gene Description
melanoma antigen family A, 9
Omim ID
300342Gene Ontology
HyperlinkGene Summary
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq
Other Designations
MAGE-9 antigen|OTTHUMP00000024214|melanoma-associated antigen 9
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Interactome
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Publication Reference
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The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28.
Rogner UC, Wilke K, Steck E, Korn B, Poustka A.
Genomics 1995 Oct; 29(3):725.
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Structure, chromosomal localization, and expression of 12 genes of the MAGE family.
De Plaen E, Arden K, Traversari C, Gaforio JJ, Szikora JP, De Smet C, Brasseur F, van der Bruggen P, Lethe B, Lurquin C, et al..
Immunogenetics 1994 Jan; 40(5):360.
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The melanoma antigen gene (MAGE) family is clustered in the chromosomal band Xq28.
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