ZBTB7B polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of ZBTB7B polyclonal antibody (Cat # PAB4466) in mouse brain tissue lysates (35 ug/lane). ZBTB7B (arrow) was detected using the purified ZBTB7B polyclonal antibody (Cat # PAB4466) (1 : 60 dilution).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ZBTB7B polyclonal antibody (Cat # PAB4466) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ZBTB7B.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to internal region of human ZBTB7B.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of ZBTB7B polyclonal antibody (Cat # PAB4466) in mouse brain tissue lysates (35 ug/lane). ZBTB7B (arrow) was detected using the purified ZBTB7B polyclonal antibody (Cat # PAB4466) (1 : 60 dilution).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma reacted with ZBTB7B polyclonal antibody (Cat # PAB4466) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .Enzyme-linked Immunoabsorbent Assay
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Gene Info — ZBTB7B
Entrez GeneID
51043Protein Accession#
NP_056956;O15156Gene Name
ZBTB7B
Gene Alias
DKFZp686G01254, THPOK, ZBTB15, ZFP67, ZNF857B, c-Krox, hcKrox
Gene Description
zinc finger and BTB domain containing 7B
Omim ID
607646Gene Ontology
HyperlinkGene Summary
ZFP67 is an early growth response gene that encodes a zinc finger-containing transcription factor that binds to the promoter regions of type I collagen genes (e.g., COL1A1; MIM 120150) and has a role in development.[supplied by OMIM
Other Designations
OTTHUMP00000035544|OTTHUMP00000035545|kruppel-related zinc finger protein hcKrox|zinc finger and BTB domain containing 15|zinc finger protein 67 homolog
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Interactome
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Publication Reference
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Functional characterization of the human biglycan 5'-flanking DNA and binding of the transcription factor c-Krox.
Heegaard AM, Gehron Robey P, Vogel W, Just W, Widom RL, Scholler J, Fisher LW, Young MF.
Journal of Bone and Mineral Research 1997 Dec; 12(12):2050.
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Cloning and characterization of hcKrox, a transcriptional regulator of extracellular matrix gene expression.
Widom RL, Culic I, Lee JY, Korn JH.
Gene 1997 Oct; 198(1-2):407.
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c-Krox, a transcriptional regulator of type I collagen gene expression, is preferentially expressed in skin.
Galera P, Musso M, Ducy P, Karsenty G.
PNAS 1994 Sep; 91(20):9372.
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Functional characterization of the human biglycan 5'-flanking DNA and binding of the transcription factor c-Krox.
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