IKBKE polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of IKBKE.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human IKBKE.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The IKBKE polyclonal antibody (Cat # PAB3999) is used in Western blot to detect IKBKE in 293 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with IKBKE polyclonal antibody (Cat # PAB3999) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
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Gene Info — IKBKE
Entrez GeneID
9641Protein Accession#
Q14164Gene Name
IKBKE
Gene Alias
IKK-i, IKKE, IKKI, KIAA0151, MGC125294, MGC125295, MGC125297
Gene Description
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
Omim ID
605048Gene Ontology
HyperlinkGene Summary
O
Other Designations
IKK-related kinase epsilon|OTTHUMP00000034540|inducible IkappaB kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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Oncogenic kinase signalling.
Blume-Jensen P, Hunter T.
Nature 2001 May; 411(6835):355.
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Phosphoinositide 3-kinase signalling pathways.
Cantrell DA.
Journal of Cell Science 2001 Apr; 114(Pt 8):1439.
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The RET proto-oncogene in human cancers.
Jhiang SM.
Oncogene 2000 Nov; 19(49):5590.
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Oncogenic kinase signalling.
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