PDGFRA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PDGFRA.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human PDGFRA.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Flow Cytometry (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of mouse lung tissue lysate (35 ug/lane) with PDGFRA polyclonal antibody (Cat # PAB3372).Western Blot (Cell lysate)
Western blot analysis of K-562 cell lysate (35 ug/lane) with PDGFRA polyclonal antibody (Cat # PAB3372).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with PDGFRA polyclonal antibody (Cat # PAB3372) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Flow Cytometry
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Gene Info — PDGFRA
Entrez GeneID
5156Protein Accession#
P16234Gene Name
PDGFRA
Gene Alias
CD140A, MGC74795, PDGFR2, Rhe-PDGFRA
Gene Description
platelet-derived growth factor receptor, alpha polypeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes. [provided by RefSeq
Other Designations
FIP1L1/PDGFRA fusion protein|platelet-derived growth factor receptor alpha|rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein
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Interactome
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Pathway
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Disease
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Publication Reference
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A tyrosine kinase created by fusion of the PDGFRA and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome.
Cools J, DeAngelo DJ, Gotlib J, Stover EH, Legare RD, Cortes J, Kutok J, Clark J, Galinsky I, Griffin JD, Cross NC, Tefferi A, Malone J, Alam R, Schrier SL, Schmid J, Rose M, Vandenberghe P, Verhoef G, Boogaerts M, Wlodarska I, Kantarjian H, Marynen P, Coutre SE, Stone R, Gilliland DG.
The New England Journal of Medicine 2003 Mar; 348(13):1201.
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PDGFRA activating mutations in gastrointestinal stromal tumors.
Heinrich MC, Corless CL, Duensing A, McGreevey L, Chen CJ, Joseph N, Singer S, Griffith DJ, Haley A, Town A, Demetri GD, Fletcher CD, Fletcher JA.
Science 2003 Jan; 299(5607):708.
Application:WB-Ti, WB-Tr, Human, Mouse, Gastrointestinal stromal tumor, CHO cells.
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Platelet-derived growth factor-B and -C and active alpha-receptors in medulloblastoma cells.
Andrae J, Molander C, Smits A, Funa K, Nister M.
Biochemical and Biophysical Research Communications 2002 Aug; 296(3):604.
Application:IP, Human, AG 1518, D283 Med, D324 Med, PFSK-1, D341 Med.
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A tyrosine kinase created by fusion of the PDGFRA and FIP1L1 genes as a therapeutic target of imatinib in idiopathic hypereosinophilic syndrome.
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