DGKZ polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
The DGKZ polyclonal antibody (Cat # PAB3265) is used in Western blot to detect DGKZ in Ramos cell lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DGKZ polyclonal antibody (Cat # PAB3265) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Immunofluorescence
SF1 colocalizes with DGKZ and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and DGKZ polyclonal antibody (Cat # PAB3265) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DGKZ.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human DGKZ.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The DGKZ polyclonal antibody (Cat # PAB3265) is used in Western blot to detect DGKZ in Ramos cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DGKZ polyclonal antibody (Cat # PAB3265) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Immunofluorescence
SF1 colocalizes with DGKZ and PA in the nuclei of H295R cells. Cells were plated onto glass coverslips, fixed, permeabilized, and incubated with anti-SF1 and DGKZ polyclonal antibody (Cat # PAB3265) for 1 h. Coverslips were washed and incubated with anti-fluorescein isothiocyanate and antirhodamine, and immunofluorescence was detected by confocal microscopy. -
Gene Info — DGKZ
Entrez GeneID
8525Protein Accession#
NP_963290;Q13574Gene Name
DGKZ
Gene Alias
DAGK5, DAGK6, DGK-ZETA, hDGKzeta
Gene Description
diacylglycerol kinase, zeta 104kDa
Omim ID
601441Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified. [provided by RefSeq
Other Designations
diacylglycerol kinase zeta|diacylglycerol kinase, zeta (104kD)
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Interactome
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Pathway
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Publication Reference
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Cyclic AMP-stimulated interaction between steroidogenic factor 1 and diacylglycerol kinase theta facilitates induction of CYP17.
Li D, Urs AN, Allegood J, Leon A, Merrill AH Jr, Sewer MB.
Molecular and Cellular Biology 2007 Jul; 27(19):6669.
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Cyclic AMP-stimulated interaction between steroidogenic factor 1 and diacylglycerol kinase theta facilitates induction of CYP17.
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