His tag polyclonal antibody (HRP)
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More Files
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Western Blot
Western blot analysis of E. coli lysates (containing tagged fusion protein) with His tag polyclonal antibody (HRP) (Cat # PAB31866).
Lane 1: 200 ng; Lane 2: 100 ng; Lane 3: 50 ng.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptides of His tag.
The antibody is conjugated with horseradish peroxidase (HRP).Immunogen
A synthetic peptide (conjugated with KLH) corresponding to His tag.
Sequence
HHHHHH
Host
Goat
Specificity
Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Form
Liquid
Conjugation
HRP
Purification
Affinity purification
Concentration
1 mg/mL
Isotype
IgG
Recommend Usage
ELISA (1:10,000 - 1:100,000)
Immunocytochemistry (1:200 - 1:500)
Immunohistochemistry
Western Blot (1:1,000 - 1:5,000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (with 0.2% BSA and 0.05% Pro-Clean 400).
Storage Instruction
Store at 2-8°C
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot analysis of E. coli lysates (containing tagged fusion protein) with His tag polyclonal antibody (HRP) (Cat # PAB31866).
Lane 1: 200 ng; Lane 2: 100 ng; Lane 3: 50 ng.Immunohistochemistry
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
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