MYLK polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot analysis of MYLK polyclonal antibody (Cat # PAB3182) in mouse brain tissue lysate. MYLK (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody (Cat # PAB3182) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MYLK.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 908-938 at N-terminus of human MYLK.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of MYLK polyclonal antibody (Cat # PAB3182) in mouse brain tissue lysate. MYLK (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody (Cat # PAB3182) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — MYLK
Entrez GeneID
4638Protein Accession#
Q9C0L5Gene Name
MYLK
Gene Alias
DKFZp686I10125, FLJ12216, KRP, MLCK, MLCK1, MLCK108, MLCK210, MSTP083, MYLK1, smMLCK
Gene Description
myosin light chain kinase
Omim ID
600922Gene Ontology
HyperlinkGene Summary
This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts. [provided by RefSeq
Other Designations
OTTHUMP00000180642|OTTHUMP00000180643|myosin, light polypeptide kinase|smooth muscle myosin light chain kinase
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Analysis of the kinase-related protein gene found at human chromosome 3q21 in a multi-gene cluster: organization, expression, alternative splicing, and polymorphic marker.
D M Watterson, J P Schavocky, L Guo, C Weiss, A Chlenski, V P Shirinsky, L J Van Eldik, J Haiech.
Journal of Cellular Biochemistry 1999 Dec; 75(3):481.
Application：IHC-P, Human, Human heart.
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Myosin light chain kinase in endothelium: molecular cloning and regulation.
Garcia JG, Lazar V, Gilbert-McClain LI, Gallagher PJ, Verin AD.
American Journal of Respiratory Cell and Molecular Biology 1997 May; 13(4):489.
Application：IP, WB-Ce, Bovine, Human, Bovine pulmonary artery endothelial cells, HUVECs.
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Analysis of the kinase-related protein gene found at human chromosome 3q21 in a multi-gene cluster: organization, expression, alternative splicing, and polymorphic marker.
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