Maltose Binding Protein tag polyclonal antibody (Biotin)
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More Files
- More Functions
Western Blot (Recombinant protein)
Western Blot analysis of Maltose Binding Protein (MBP) (0.05 ug) with Maltose Binding Protein tag polyclonal antibody (Biotin) (Cat # PAB31806).
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant MBP epitope tag. The antibody is conjugated with Biotin.
Immunogen
Recombinant protein corresponding to MBP epitope tag.
Host
Rabbit
Specificity
This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
Form
Lyophilized
Conjugation
Biotin
Purification
IgG purification
Isotype
IgG
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) sodium azide).
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western Blot analysis of Maltose Binding Protein (MBP) (0.05 ug) with Maltose Binding Protein tag polyclonal antibody (Biotin) (Cat # PAB31806).Enzyme-linked Immunoabsorbent Assay
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