HA tag polyclonal antibody (Biotin)
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More Files
- More Functions
Western Blot (Recombinant protein)
Western Blot analysis of recombinant transcription factor protein containing the HA epitope tag (1.0 ug) with HA tag polyclonal antibody (Biotin) (Cat # PAB31802) at 1:10000 working concentration.
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Specification
Product Description
Rabbit polyclonal antibody raised against HA epitope tag peptide. The antibody is conjugated with Biotin.
Immunogen
A epitope tag peptide (conjugated with KLH) corresponding to YPYDVPDYA from hemagglutinin influenza.
Sequence
YPYDVPDYA
Host
Rabbit
Specificity
This affinity purified antibody is directed against the HA motif and is useful in determining its presence in various assays. This polyclonal anti-HA tag antibody detects over-expressed proteins containing the HA epitope tag. To date this antibody has reacted with all HA tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-rabbit serum.
Form
Lyophilized
Conjugation
Biotin
Preparation Method
This antibody was purified from whole rabbit serum prepared by repeated immunizations with the 9-aa epitope tag peptide YPYDVPDYA (11 114-122) from hemagglutinin influenza conjugated to KLH using maleimide. A residue of cysteine was added to the carboxy terminal end to facilitate coupling.
Purification
Affinity purification
Isotype
IgG
Conjugation Note
FP Value: 10-20 moles Biotin per mole of IgG
Recommend Usage
ELISA (1:10000-1:50000)
Immunohistochemistry (1:1000-1:5000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM potassium phosphate, 150 mM NaCl, pH 7.2 (10 mg/mL BSA, 0.01% (w/v) sodium azide).
Storage Instruction
Store vial at 4°C prior to restoration.
After reconstitution with 100 uL deionized water (or equivalent), aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western Blot analysis of recombinant transcription factor protein containing the HA epitope tag (1.0 ug) with HA tag polyclonal antibody (Biotin) (Cat # PAB31802) at 1:10000 working concentration.Immunohistochemistry
Enzyme-linked Immunoabsorbent Assay
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