ACAT2 polyclonal antibody
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: RT-4 cell, Lane 2: U-251 MG sp cell, Lane 3: human plasma tissue (IgG/HSA depleted), Lane 4: human liver tissue and Lane 5: human tonsil tissue lysates with ACAT2 polyclonal antibody (Cat # PAB31547).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with ACAT2 polyclonal antibody (Cat # PAB31547) shows strong cytoplasmic positivity in Leydig cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human ACAT2.
Immunogen
Recombinant protein corresponding to human ACAT2.
Sequence
SIGIGDSSIVVAGGMENMSKAPHLAYLRTGVKIGEMPLTDSILCDGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: RT-4 cell, Lane 2: U-251 MG sp cell, Lane 3: human plasma tissue (IgG/HSA depleted), Lane 4: human liver tissue and Lane 5: human tonsil tissue lysates with ACAT2 polyclonal antibody (Cat # PAB31547).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with ACAT2 polyclonal antibody (Cat # PAB31547) shows strong cytoplasmic positivity in Leydig cells. -
Gene Info — ACAT2
Entrez GeneID
39Protein Accession#
Q9BWD1Gene Name
ACAT2
Gene Alias
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Gene Description
acetyl-Coenzyme A acetyltransferase 2
Omim ID
100678Gene Ontology
HyperlinkGene Summary
The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq
Other Designations
OTTHUMP00000017527|acetoacetyl Coenzyme A thiolase|cytosolic acetoacetyl-CoA thiolase
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Interactome
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Pathway
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Disease
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