PI15 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human PI15.
Immunogen
Recombinant protein corresponding to human PI15.
Sequence
LLFSLLCEASTVVLLNSTDSSPPTNNFTDIEAALKAQLDSADIPKARRKRYISQNDMIAILDYHNQVRGKVFPPAANMEYMVWDENLAKSAEAWAATCIWDHGPSYLLRFLGQNLSVRTGRYRSILQLVKPWYDEVKDY
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with PI15 polyclonal antibody (Cat # PAB31508).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human lung with PI15 polyclonal antibody (Cat # PAB31508) shows strong cytoplasmic positivity in alveolar macrophages. -
Gene Info — PI15
Entrez GeneID
51050Protein Accession#
O43692Gene Name
PI15
Gene Alias
CRISP8, DKFZp686F0366, P24TI, P25TI
Gene Description
peptidase inhibitor 15
Omim ID
607076Gene Ontology
HyperlinkGene Summary
This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq
Other Designations
25 kDa trypsin inhibitor|protease inhibitor 15
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