AGR2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human AGR2.
Immunogen
Recombinant protein corresponding to human AGR2.
Sequence
RDTTVKPGAKKDTKDSRPKLPQTLSRGWGDQLIWTQTYEEALYKSKTSNKPLMIIHHLDECPHSQALKKVFAENKEIQKLAEQFVLLNLVYETTDKHLSPDGQYVPRIMFVDPSLTVRADITGR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:500)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of A-549 cell lysate with AGR2 polyclonal antibody (Cat # PAB31396).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach with AGR2 polyclonal antibody (Cat # PAB31396) shows strong cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of RT-4 cells with AGR2 polyclonal antibody (Cat # PAB31396) (Green) shows localization to cytosol. -
Gene Info — AGR2
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Interactome
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Disease
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Publication Reference
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AGR2 predicts tamoxifen resistance in postmenopausal breast cancer patients.
Hrstka R, Brychtova V, Fabian P, Vojtesek B, Svoboda M.
Disease Markers 2013 Sep; 35(4):207.
Application:IHC-P, Human, Breast cancer.
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Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells.
Charlotte Stadler, Elton Rexhepaj, Vasanth R Singan, Robert F Murphy, Rainer Pepperkok, Mathias Uhlén, Jeremy C Simpson, Emma Lundberg.
Nature Methods 2013 Apr; 10(4):315.
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AGR2 predicts tamoxifen resistance in postmenopausal breast cancer patients.
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