PRMT3 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with PRMT3 polyclonal antibody (Cat # PAB31394).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with PRMT3 polyclonal antibody (Cat # PAB31394) shows nuclear positivity in cells in seminiferous ducts.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human PRMT3.
Immunogen
Recombinant protein corresponding to human PRMT3.
Sequence
TFSHCKSEHQFNIDSMVHKHGLEFYGYIKLINFIRLKNPTVEYMNSIYNPVPWEKEEYLKPVLEDDLLLQFDVEDLYEPVSVPFSYPNGLSENTSVVEKLKHMEARALSAEAALARAREDLQKMKQFAQDFVMHTDVRTCSSS
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with PRMT3 polyclonal antibody (Cat # PAB31394).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with PRMT3 polyclonal antibody (Cat # PAB31394) shows nuclear positivity in cells in seminiferous ducts. -
Gene Info — PRMT3
Entrez GeneID
10196Protein Accession#
O60678Gene Name
PRMT3
Gene Alias
HRMT1L3
Gene Description
protein arginine methyltransferase 3
Omim ID
603190Gene Ontology
HyperlinkGene Summary
Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM
Other Designations
HMT1 hnRNP methyltransferase-like 3|heterogeneous nuclear ribonucleoprotein methyltransferase-like 3|protein arginine N-methyltransferase 3
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Interactome
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Disease
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