EGFP polyclonal antibody
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More Files
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Immunofluorescence
Immunofluorescent staining of mitotic Hela cell line stably expressing a GFP-CENP-A fusion protein followed by antibody against EGFP (left). And an monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody (middle). DNA was visualized using Hoechst staining (right).
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Specification
Product Description
Goat polyclonal antibody raised against EGFP.
Immunogen
Recombinant protein corresponding to full length His-tagged EGFP.
Host
Goat
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Recommend Usage
Immunofluorescence (1:800)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunofluorescence
Immunofluorescent staining of mitotic Hela cell line stably expressing a GFP-CENP-A fusion protein followed by antibody against EGFP (left). And an monoclonal antibody against HEC1, followed by a CY2-labeled anti-goat and a CY3-labeled anti-mouse antibody (middle). DNA was visualized using Hoechst staining (right).
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