SAP30 polyclonal antibody
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More Files
- More Functions
ChIP
ChIP assays were performed using HeLa cells. A titration of the antibody consisting of 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP-Seq
ChIP was performed on sheared chromatin from 4 million HeLa cells using 5 ug antibody. The figure shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X.
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 20 ug nuclear extracts of HeLa cells.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant SAP30.
Immunogen
Recombinant His-tag fusion protein corresponding to human SAP30.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
ChIP (2-5 ug/CHIP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300).
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP assays were performed using HeLa cells. A titration of the antibody consisting of 1, 2 and 5 ug per ChIP experiment was analysed. IgG (1 ug/ IP) was used as negative IP control. QPCR was performed with primers for the EIF2S3 and BRCA1 promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).ChIP-Seq
ChIP was performed on sheared chromatin from 4 million HeLa cells using 5 ug antibody. The figure shows the enrichment along the complete sequence and a 1.5 Mb region of human chromosome 1 and in two genomic regions surrounding the BRCA1 and EIF2S3 genes on chromosome 17 and X.Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of 20 ug nuclear extracts of HeLa cells. -
Gene Info — SAP30
Entrez GeneID
8819Protein Accession#
O75446Gene Name
SAP30
Gene Alias
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Gene Description
Sin3A-associated protein, 30kDa
Omim ID
603378Gene Ontology
HyperlinkGene Summary
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq
Other Designations
Sin3 corepressor complex subunit SAP30|Sin3-associated polypeptide, 30kDa|histone deacetylase complex subunit SAP30
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