EML4 polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against partial recombinant human EML4.
Immunogen
Recombinant protein corresponding to human EML4.
Sequence
MSIIQWKLVEKLSLPQNETVADTTLTKAPVSSTESVIQSNTPTPPPSQPLNETAEEESRISSSPTLLENSLEQTVEPSEDHSEEES
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:1000-2500)
Western Blot (1:100-250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot (Cell lysate) analysis of RT-4 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of U-251MG cell line with antibody shows positivity in cytosol, intermediate filaments and microtubules (green). -
Gene Info — EML4
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Interactomes
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Diseases
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Publication Reference
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Neural differentiation modulates the vertebrate brain specific splicing program.
Madgwick A, Fort P, Hanson PS, Thibault P, Gaudreau MC, Lutfalla G, Moroy T, Abou Elela S, Chaudhry B, Elliott DJ, Morris CM, Venables JP.
PLoS One 2015 May; 10(5):e0125998.
Application:WB-Ce, Human, Human neuronal precursor stem cell.
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Neural differentiation modulates the vertebrate brain specific splicing program.
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