MARS polyclonal antibody

Catalog # PAB30671

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Size:100 uL
Price: USD $ 428.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with MARS polyclonal antibody (Cat # PAB30671).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex (A, B), human small intestine (C) with MARS polyclonal antibody (Cat # PAB30671). A: Human cerebral cortex shows cytoplasmic positivity in subsets of neurons. B: Human cerebral cortex shows cytoplasmic immunoreactivity in neuronal cell bodies. C: Human small intestine shows moderate cytoplasmic positivity in glandular cells.

Immunofluorescence
Application

Immunofluorescence

Immunofluorescent staining of U-2 OS (A), mouse cerebellum (B), mouse olfactory bulb (C), mouse visual cortex (D), mouse cerebral peduncle (E) and mouse pons (F) with MARS polyclonal antibody (Cat # PAB30671) (Green). A: U-2 OS shows positivity in cytoplasm. B: Mouse cerebellum shows cytoplasmic immunoreactivity in Purkinje cells. C: Mouse olfactory bulb shows cytoplasmic positivity in mitral and external plexiform layer neurons. D: Mouse visual cortex shows cytoplasmic and axonal staining in neurons. E: Mouse cerebral peduncle shows cytoplasmic positivity in red nucleus neurons. F: Mouse pons shows neuronal positivity in motor trigeminal nucleus.

  • Specification

    Product Description

    Rabbit polyclonal antibody raised against partial recombinant human MARS.

    Immunogen

    Recombinant protein corresponding to human MARS.

    Sequence

    FVLQDTVEQLRCEHCARFLADRFVEGVCPFCGYEEARGDQCDKCGKLINAVELKKPQCKVCRSCPVVQSSQHLFLDLPKLEKRLEEWLGRTLPGSDWTPNAQFITRSWLRDGLKPRCITRDLKWGTPVPLEGFEDKVFYVWFD

    Host

    Rabbit

    Reactivity

    Human, Mouse

    Form

    Liquid

    Purification

    Antigen affinity purification

    Isotype

    IgG

    Recommend Usage

    Immunofluorescence (1-4 ug/mL)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
    Western Blot (1:100-1:250)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western Blot analysis of Lane 1: RT-4 and Lane 2: U-251MG sp cell lysates with MARS polyclonal antibody (Cat # PAB30671).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex (A, B), human small intestine (C) with MARS polyclonal antibody (Cat # PAB30671). A: Human cerebral cortex shows cytoplasmic positivity in subsets of neurons. B: Human cerebral cortex shows cytoplasmic immunoreactivity in neuronal cell bodies. C: Human small intestine shows moderate cytoplasmic positivity in glandular cells.

    Immunofluorescence

    Immunofluorescent staining of U-2 OS (A), mouse cerebellum (B), mouse olfactory bulb (C), mouse visual cortex (D), mouse cerebral peduncle (E) and mouse pons (F) with MARS polyclonal antibody (Cat # PAB30671) (Green). A: U-2 OS shows positivity in cytoplasm. B: Mouse cerebellum shows cytoplasmic immunoreactivity in Purkinje cells. C: Mouse olfactory bulb shows cytoplasmic positivity in mitral and external plexiform layer neurons. D: Mouse visual cortex shows cytoplasmic and axonal staining in neurons. E: Mouse cerebral peduncle shows cytoplasmic positivity in red nucleus neurons. F: Mouse pons shows neuronal positivity in motor trigeminal nucleus.
  • Gene Info — MARS

    Entrez GeneID

    4141

    Protein Accession#

    P56192

    Gene Name

    MARS

    Gene Alias

    FLJ35667, METRS, MTRNS

    Gene Description

    methionyl-tRNA synthetase

    Omim ID

    156560

    Gene Ontology

    Hyperlink

    Gene Summary

    Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene belongs to the class I family of tRNA synthetases. [provided by RefSeq

    Other Designations

    methionine tRNA ligase 1, cytoplasmic|methionine-tRNA synthetase

  • Interactome
  • Pathway
  • Disease
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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