JAM3 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with JAM3 polyclonal antibody (Cat # PAB30620).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with JAM3 polyclonal antibody (Cat # PAB30620) shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunofluorescence
Immunofluorescent staining of U-251 MG with JAM3 polyclonal antibody (Cat # PAB30620) (Green) shows positivity in the Golgi apparatus.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human JAM3.
Immunogen
Recombinant protein corresponding to human JAM3.
Sequence
RDSALYRCEVVARNDRKEIDEIVIELTVQVKPVTPVCRVPKAVPVGKMATLHCQESEGHPRPHYSWYRNDVPLPTDSRANPRFRNSSFHLNSETGTLVFTAVHKDDSGQYYCIASNDAGSARCEEQEMEVYDLNIG
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: negative control (vector only transfected HEK293T cell lysate) and Lane 2: over-expression lysate (co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells) with JAM3 polyclonal antibody (Cat # PAB30620).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testis with JAM3 polyclonal antibody (Cat # PAB30620) shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.Immunofluorescence
Immunofluorescent staining of U-251 MG with JAM3 polyclonal antibody (Cat # PAB30620) (Green) shows positivity in the Golgi apparatus. -
Gene Info — JAM3
Entrez GeneID
83700Protein Accession#
Q9BX67Gene Name
JAM3
Gene Alias
FLJ14529, JAM-C, JAMC
Gene Description
junctional adhesion molecule 3
Omim ID
606871Gene Ontology
HyperlinkGene Summary
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. [provided by RefSeq
Other Designations
junctional adhesion molecule C
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Interactome
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Pathway
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Disease
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