ERBB3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ERBB3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human ERBB3.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Flow cytometry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of ERBB3 polyclonal antibody (Cat # PAB3037) in mouse brain lysate. ERBB3 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ERBB3 polyclonal antibody (Cat # PAB3037) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Flow Cytometry
Flow cytometric analysis of 293 cells using ERBB3 polyclonal antibody (Cat # PAB3037)(bottom histogram) compared to a negative control cell (top histogram).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. -
Gene Info — ERBB3
Entrez GeneID
2065Protein Accession#
P21860Gene Name
ERBB3
Gene Alias
ErbB-3, HER3, LCCS2, MDA-BF-1, MGC88033, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3
Gene Description
v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq
Other Designations
erbB-3|lethal congenital contracture syndrome 2|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
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Interactome
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Pathway
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Disease
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Publication Reference
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Bone morphogenetic proteins regulate enteric gliogenesis by modulating ErbB3 signaling.
Chalazonitis A, D'Autreaux F, Pham TD, Kessler JA, Gershon MD.
Developmental Biology 2011 Feb; 350(1):64.
Application:ICC, Mouse, Intestine.
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c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.
Katoh M, Yazaki Y, Sugimura T, Terada M.
Biochemical and Biophysical Research Communications 1993 May; 192(3):1189.
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Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.
Plowman GD, Whitney GS, Neubauer MG, Green JM, McDonald VL, Todaro GJ, Shoyab M.
PNAS 1990 Jul; 87(13):4905.
Application:WB-Tr, Monkey, COS cells.
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Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors.
Kraus MH, Issing W, Miki T, Popescu NC, Aaronson SA.
PNAS 1989 Dec; 86(23):9193.
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Bone morphogenetic proteins regulate enteric gliogenesis by modulating ErbB3 signaling.
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