PDIA3 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251 MG sp; Lane 3: Human liver tissue; Lane 4: Human tonsil tissue with PDIA3 polyclonal antibody (Cat# PAB29335) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with PDIA3 polyclonal antibody (Cat# PAB29335) shows strong cytoplasmic positivity in glandular cells at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with PDIA3 polyclonal antibody (Cat# PAB29335) under 1-4 ug/mL working concentration shows positivity in endoplasmic reticulum.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human PDIA3.
Immunogen
Recombinant protein corresponding to amino acids of human PDIA3.
Sequence
HMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGP
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:500-1:1000)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Human cell line RT-4; Lane 2: Human cell line U-251 MG sp; Lane 3: Human liver tissue; Lane 4: Human tonsil tissue with PDIA3 polyclonal antibody (Cat# PAB29335) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human stomach with PDIA3 polyclonal antibody (Cat# PAB29335) shows strong cytoplasmic positivity in glandular cells at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with PDIA3 polyclonal antibody (Cat# PAB29335) under 1-4 ug/mL working concentration shows positivity in endoplasmic reticulum. -
Gene Info — PDIA3
Entrez GeneID
2923Protein Accession#
G5EA52Gene Name
PDIA3
Gene Alias
ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC
Gene Description
protein disulfide isomerase family A, member 3
Omim ID
602046Gene Ontology
HyperlinkGene Summary
This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq
Other Designations
58 kDa microsomal protein|OTTHUMP00000041709|endoplasmic reticulum P58|glucose regulated protein, 58kDa|phospholipase C-alpha|protein disulfide isomerase-associated 3|protein disulfide-isomerase A3
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Interactome
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Pathway
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Disease
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