SNAI1 (phospho S246) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HT-29 cells, Lane 2: antigen-specific peptide treated HT-29 cells with SNAI1 (phospho S246) polyclonal antibody (Cat# PAB29288) at 1:500-1:1000 dilution.
Immunofluorescence
Immunofluorescence staining of methanol-fixed HUVEC cells with SNAI1 (phospho S246) polyclonal antibody (Cat# PAB29288) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human SNAI1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S246 of human SNAI1.
Host
Rabbit
Theoretical MW (kDa)
29
Reactivity
Human, Mouse
Specificity
SNAI1 (phospho S246) polyclonal antibody detects endogenous levels of human SNAI1 only when phosphorylated at serine 246.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunofluorescence (1:100~1:200)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HT-29 cells, Lane 2: antigen-specific peptide treated HT-29 cells with SNAI1 (phospho S246) polyclonal antibody (Cat# PAB29288) at 1:500-1:1000 dilution.Immunofluorescence
Immunofluorescence staining of methanol-fixed HUVEC cells with SNAI1 (phospho S246) polyclonal antibody (Cat# PAB29288) without blocking peptide (A) or preincubated with blocking peptide (B) at 1:100-1:200 dilution. -
Gene Info — SNAI1
Entrez GeneID
6615Protein Accession#
O95863Gene Name
SNAI1
Gene Alias
SLUGH2, SNA, SNAH, dJ710H13.1
Gene Description
snail homolog 1 (Drosophila)
Omim ID
604238Gene Ontology
HyperlinkGene Summary
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq
Other Designations
OTTHUMP00000031680|snail 1 homolog|snail 1 zinc finger protein|snail 1, zinc finger protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-β/epithelial-mesenchymal transition signaling pathway.
Yuan CL, Liang R, Liu ZH, Li YQ, Luo XL, Ye JZ, Lin Y.
Experimental and Therapeutic Medicine 2018 Jun; 15(6):5422.
Application：WB-Tr, Human, BGC‑823 cells.
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Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-β/epithelial-mesenchymal transition signaling pathway.
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