CEBPA (phospho S21) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: EGF treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with CEBPA (phospho S21) polyclonal antibody (Cat# PAB29256) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer tissue with CEBPA (phospho S21) polyclonal antibody (Cat# PAB29256) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human CEBPA.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S21 of human CEBPA.
Host
Rabbit
Theoretical MW (kDa)
45
Reactivity
Human, Mouse, Rat
Specificity
CEBPA (phospho S21) polyclonal antibody detects endogenous levels of human CEBPA only when phosphorylated at serine 21.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: EGF treated HepG2 cells, Lane 2: antigen-specific peptide treated HepG2 cells with CEBPA (phospho S21) polyclonal antibody (Cat# PAB29256) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human colon cancer tissue with CEBPA (phospho S21) polyclonal antibody (Cat# PAB29256) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — CEBPA
Entrez GeneID
1050Protein Accession#
P49715Gene Name
CEBPA
Gene Alias
C/EBP-alpha, CEBP
Gene Description
CCAAT/enhancer binding protein (C/EBP), alpha
Gene Ontology
HyperlinkGene Summary
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq
Other Designations
CCAAT/enhancer binding protein alpha
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com