SYT1 (phospho T202) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated COLO205 cells, Lane 2: COLO205 cells with SYT1 (phospho T202) polyclonal antibody (Cat# PAB29255) at 1:500-1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with SYT1 (phospho T202) polyclonal antibody (Cat# PAB29255) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human SYT1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T202 of human SYT1.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Rat
Specificity
SYT1 (phospho T202) polyclonal antibody detects endogenous levels of human SYT1 only when phosphorylated at threonine 202.
Form
Liquid
Purification
Affinity Chromatography
Recommend Usage
Immunohistochemistry (1:50~1:100)
Western Blot (1:500~1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: antigen-specific peptide treated COLO205 cells, Lane 2: COLO205 cells with SYT1 (phospho T202) polyclonal antibody (Cat# PAB29255) at 1:500-1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer tissue with SYT1 (phospho T202) polyclonal antibody (Cat# PAB29255) without blocking peptide (A) or preincubated with blocking peptide (B) under 1:50-1:100 dilution. -
Gene Info — SYT1
Entrez GeneID
6857Protein Accession#
P21579Gene Name
SYT1
Gene Alias
DKFZp781D2042, P65, SVP65, SYT
Gene Description
synaptotagmin I
Omim ID
185605Gene Ontology
HyperlinkGene Summary
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM
Other Designations
OTTHUMP00000166064|synaptotagmin 1
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Interactome
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Disease
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