FLAG polyclonal antibody
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More Files
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Specification
Product Description
Chicken polyclonal antibody raised against recombinant FLAG peptide.
Immunogen
A KLH-conjugated synthetic peptide corresponding to Flag tag emulsified in Freund's adjuvant.
Sequence
DYKDDDDK
Host
Chicken
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgY
Recommend Usage
ELISA
Immunocytochemistry(1:500)
Immunohistochemistry
Immunoprecipitation(1:500)
Western Blot(1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.02% sodium azide)
Storage Instruction
Store at 4°C and avoid from light. For long term storage, dilute the antibody with sterile glycerol and store it at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Cell cycle specific, differentially tagged ribosomal proteins to measure phase specific transcriptomes from asynchronously cycling cells.
Jesse D Cochran, Tess A Leathers, Emir Maldosevic, Klara W Siejda, Julian Vitello, Haesol Lee, Leigh A Bradley, Alex Young, Ahmad Jomaa, Matthew J Wolf.
Scientific Reports 2024 Jan; 14(1):1623.
Application：IF, Human, HEK-293T cell line.
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Yeast biopanning against site-specific phosphorylations in tau.
Monika Arbaciauskaite, Azady Pirhanov, Erik Ammermann, Yu Lei, Yong Ku Cho.
Protein Engineering, Design & Selection : PEDS 2023 Jan; 36:gzad005.
Application：Flow Cyt, Yeast, Yeast.
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Porcine 2', 5'-oligoadenylate synthetase 2 inhibits porcine reproductive and respiratory syndrome virus replication in vitro.
Zhao M, Wan B, Li H, He J, Chen X, Wang L, Wang Y, Xie S, Qiao S, Zhang G.
Microbial Pathogenesis 2017 Aug; [Epub].
Application：WB, Pig, Porcine alveolar macrophages.
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Cell cycle specific, differentially tagged ribosomal proteins to measure phase specific transcriptomes from asynchronously cycling cells.
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