Actin polyclonal antibody
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Specification
Product Description
Chicken polyclonal antibody raised against purified Rabbit Actin.
Immunogen
Purified rabbit muscle actin.
Host
Chicken
Reactivity
Human, Mouse, Rabbit, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgY
Quality Control Testing
This antibody was validated by western blot with nitrocellulose membranes (at a concentration of 1 ug/mL) using HRP-labeled goat anti-chicken IgY (1:5000 dilution) as the secondary reagent.
Do NOT use this product with PVDF, or other nylon-based membranes, because this often produces unacceptably high backgrounds in chemiluminescence studies.Recommend Usage
Immunocytochemistry
Western Blot(1:2500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C and avoid from light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunocytochemistry
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Publication Reference
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Protein tyrosine phosphatase receptor-type O expression as a prognostic marker in patients with acute coronary syndrome undergoing percutaneous coronary intervention: A prospective study.
Minghui Hao, Ming Guo, Rui Yan.
Experimental and Therapeutic Medicine 2021 May; 21(5):435.
Application：WB-Ce, Human, Human PBMCs.
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Protein tyrosine phosphatase receptor-type O expression as a prognostic marker in patients with acute coronary syndrome undergoing percutaneous coronary intervention: A prospective study.
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