NCAPH polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant NCAPH.
Immunogen
Recombinant protein corresponding to amino acids of human NCAPH.
Sequence
KFTNTQITEHYSTCIKLSTENKITTKNAFGLHLIDFMSEILKQKDTEPTNFKVAAGTLDASTKIYAVRVDAVHADVYRVLGGLGKDAPSLEEVEGHVADGSATEMGTTKKAVKPKKKHLHRTIEQNINNLNVSEADRKCEI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp with NCAPH polyclonal antibody (Cat # PAB28628) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human heart with NCAPH polyclonal antibody (Cat # PAB28628) shows strong cytoplasmic positivity in myocytes at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with NCAPH polyclonal antibody (Cat # PAB28628) at 1-4 ug/ml shows positivity in cytoplasm. -
Gene Info — NCAPH
Entrez GeneID
23397Protein Accession#
C9J470Gene Name
NCAPH
Gene Alias
BRRN1, CAP-H, HCAP-H
Gene Description
non-SMC condensin I complex, subunit H
Omim ID
602332Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. [provided by RefSeq
Other Designations
XCAP-H homolog|barren homolog 1|chromosome-associated protein H|condensin complex subunit 2
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Interactome
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