HS3ST1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant HS3ST1.
Immunogen
Recombinant protein corresponding to human HS3ST1.
Sequence
TQVFYNHMQKHKPYPSIEEFLVRDGRLNVDYKALNRSLYHVHMQNWLRFFPLRHIHIVDGDRLIRDPFPEIQKVERFLKLSPQINASNFYFNKTKGFYCLRDSGRDRCLHESKGRAHPQVDPKLLNKLHEYFHEPNKKFFELVGR
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:250)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:20-1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of HEK293T cell lysate using HS3ST1 polyclonal antibody (Cat # PAB28581).
Lane 1: Negative control (vector only)
Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human lateral ventricle wall with HS3ST1 polyclonal antibody (Cat # PAB28581) shows strong cytoplasmic positivity in neuronal cells. Retrieval method: HIER pH6 -
Gene Info — HS3ST1
Entrez GeneID
9957Protein Accession#
O14792Gene Name
HS3ST1
Gene Alias
3OST, 3OST1
Gene Description
heparan sulfate (glucosamine) 3-O-sulfotransferase 1
Omim ID
603244Gene Ontology
HyperlinkGene Summary
Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. The enzyme encoded by this gene is a member of the heparan sulfate biosynthetic enzyme family. It possesses both heparan sulfate glucosaminyl 3-O-sulfotransferase activity, anticoagulant heparan sulfate conversion activity, and is a rate limiting enzyme for synthesis of anticoagulant heparan. This enzyme is an intraluminal Golgi resident protein. [provided by RefSeq
Other Designations
heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1|heparin-glucosamine 3-O-sulfotransferase
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