KIAA0020 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with KIAA0020 polyclonal antibody (PAB28555) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast with KIAA0020 polyclonal antibody (Cat # PAB28555) shows nuclear membranous positivity in glandular cells at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of A-431 with KIAA0020 polyclonal antibody (Cat # PAB28555) at 1-4 ug/mL dilution shows positivity in nucleoli.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant KIAA0020.
Immunogen
Recombinant protein corresponding to amino acids of recombinant KIAA0020.
Sequence
EHAQEVVLDKSACVLVSDILGSATGDVQPTMNAIASLAATGLHPGGKDGELHIAEHPAGHLVLKWLIEQDKKMKENGREGCFAKTLVEHVGMKNLKSWASVNRGAIILSSLLQSCDLEVANKVKAALKSLIPTLEKTKSTSKGI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry(1:50-1:200)
Western Blot(1:100-1:250)
Immunofluorescence (1-4 ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with KIAA0020 polyclonal antibody (PAB28555) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human breast with KIAA0020 polyclonal antibody (Cat # PAB28555) shows nuclear membranous positivity in glandular cells at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of A-431 with KIAA0020 polyclonal antibody (Cat # PAB28555) at 1-4 ug/mL dilution shows positivity in nucleoli. -
Gene Info — KIAA0020
Entrez GeneID
9933Protein Accession#
Q15397Gene Name
KIAA0020
Gene Alias
HLA-HA8, MGC8749, PEN, PUF6, XTP5
Gene Description
KIAA0020
Omim ID
609960Gene Ontology
HyperlinkOther Designations
HBV X-transactivated gene 5 protein|KIAA0020 protein|minor histocompatibility antigen HA-8|penguin homolog|protein 5 transactivated by hepatitis B virus X antigen (HBxAg)
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Interactome
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Disease
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