PLOD3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant PLOD3.
Immunogen
Recombinant protein corresponding to human PLOD3.
Sequence
FDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEAL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1:100-1:250)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4 °C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of U-251 MG cell lysate using PLOD3 polyclonal antibody (Cat # PAB28401).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human appendix with PLOD3 polyclonal antibody (Cat # PAB28401) shows moderate cytoplasmic positivity in glandular cells. -
Gene Info — PLOD3
Entrez GeneID
8985Protein Accession#
O60568Gene Name
PLOD3
Gene Alias
LH3
Gene Description
procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Omim ID
603066Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. [provided by RefSeq
Other Designations
lysine hydroxylase 3|lysyl hydroxylase 3
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Interactome
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Pathway
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Disease
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