BRAP polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with BRAP polyclonal antibody (Cat # PAB27873) at 1:100-1:250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with BRAP polyclonal antibody (Cat # PAB27873) shows strong cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with BRAP polyclonal antibody (Cat # PAB27873) at 1-4 ug/mL dilution shows positivity in nuclear membrane & cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant BRAP.
Immunogen
Recombinant protein corresponding to amino acids of human BRAP.
Sequence
SLVVIRLELAEHSPVPAGFGFSAAAGEMSDEEIKKTTLASAVACLEGKSPGEKVAIIHQHLGRREMTDVIIETMKSNPDELKTTVEERKSSEA
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Western Blot (1:100-1:250)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2, (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with BRAP polyclonal antibody (Cat # PAB27873) at 1:100-1:250 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human testis with BRAP polyclonal antibody (Cat # PAB27873) shows strong cytoplasmic positivity in cells in seminiferus ducts at 1:50-1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line U-2 OS with BRAP polyclonal antibody (Cat # PAB27873) at 1-4 ug/mL dilution shows positivity in nuclear membrane & cytoplasm. -
Gene Info — BRAP
Entrez GeneID
8315Gene Name
BRAP
Gene Alias
BRAP2, IMP, RNF52
Gene Description
BRCA1 associated protein
Omim ID
604986Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. [provided by RefSeq
Other Designations
impedes mitogenic signal propagation
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Interactome
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Disease
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