UBASH3A polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of UBASH3A.
Immunogen
A synthetic peptide corresponding to amino acids 470-482 at internal region of human UBASH3A.
Sequence
DSGIRISSVFASP
Host
Goat
Theoretical MW (kDa)
70
Reactivity
Human
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (1-3ug/ml)
Immunohistochemistry (3-5ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
UBASH3A polycolnal antibody (Cat # PAB27601) (1ug/ml) staining of human spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
UBASH3A polycolnal antibody (Cat # PAB27601) (3.8ug/ml) staining of paraffin embedded human breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. -
Gene Info — UBASH3A
Entrez GeneID
53347Protein Accession#
NP_061834.1;NP_001001895.1Gene Name
UBASH3A
Gene Alias
CLIP4, STS-2, TULA
Gene Description
ubiquitin associated and SH3 domain containing, A
Omim ID
605736Gene Ontology
HyperlinkGene Summary
O
Other Designations
T-cell ubiquitin ligand protein
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Interactome
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Disease
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