TPO polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
TPO polycolnal antibody (Cat # PAB27581) (0.1ug/ml) staining of thyroid gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TPO.
Immunogen
A synthetic peptide corresponding to C-terminus of human TPO.
Sequence
ELRCGKHQAVGTSPQ
Host
Goat
Theoretical MW (kDa)
110
Reactivity
Human
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.1-0.3ug/ml)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
TPO polycolnal antibody (Cat # PAB27581) (0.1ug/ml) staining of thyroid gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. -
Gene Info — TPO
Entrez GeneID
7173Protein Accession#
NP_000538.3;NP_783650.1;NP_783652.1;NP_783653.1Gene Name
TPO
Gene Alias
MSA, TPX
Gene Description
thyroid peroxidase
Gene Ontology
HyperlinkGene Summary
This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000115304|OTTHUMP00000115536|thyroid microsomal antigen|thyroperoxidase
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Pathway
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Disease
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