UCK2 polyclonal antibody
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More Files
- More Functions
Western Blot (Transfected lysate)
Western blot analysis of UCK2 (arrow) using UCK2 polyclonal antibody (Cat # PAB2755).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCK2 gene (Lane 2).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UCK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UCK2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of UCK2 (arrow) using UCK2 polyclonal antibody (Cat # PAB2755).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UCK2 gene (Lane 2).Immunohistochemistry
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Gene Info — UCK2
Entrez GeneID
7371Protein Accession#
NP_036606;Q9BZX2Gene Name
UCK2
Gene Alias
TSA903, UK, UMPK
Gene Description
uridine-cytidine kinase 2
Omim ID
609329Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene catalyzes the phosphorylation of uridine monophosphate to uridine diphosphate. This is the first step in the production of the pyrimidine nucleoside triphosphates required for RNA and DNA synthesis. In addition, an allele of this gene may play a role in mediating nonhumoral immunity to Hemophilus influenzae type B. [provided by RefSeq
Other Designations
OTTHUMP00000038005|uridine kinase|uridine monophosphate kinase
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Interactome
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Pathway
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Disease
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Publication Reference
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A crucial role of uridine/cytidine kinase 2 in antitumor activity of 3'-ethynyl nucleosides.
Murata D, Endo Y, Obata T, Sakamoto K, Syouji Y, Kadohira M, Matsuda A, Sasaki T.
Drug Metabolism and Disposition 2004 Oct; 32(10):1178.
Application：WB-Ce, Human, HT-1080 cells.
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Reaction of human UMP-CMP kinase with natural and analog substrates.
Pasti C, Gallois-Montbrun S, Munier-Lehmann H, Veron M, Gilles AM, Deville-Bonne D.
European Journal of Biochemistry 2003 Apr; 270(8):1784.
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Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates.
Liou JY, Dutschman GE, Lam W, Jiang Z, Cheng YC.
Cancer Research 2002 Mar; 62(6):1624.
Application：IF, WB, Human, HeLa cells.
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A crucial role of uridine/cytidine kinase 2 in antitumor activity of 3'-ethynyl nucleosides.
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