PGK2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PGK2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human PGK2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PGK2 polyclonal antibody (Cat # PAB2727) in HepG2 cell line lysate (35 ug/lane). PGK2 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PGK2 polyclonal antibody (Cat # PAB2727) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — PGK2
Entrez GeneID
5232Protein Accession#
NP_620061;P07205Gene Name
PGK2
Gene Alias
PGK-2, PGKB, PGKPS, dJ417L20.2
Gene Description
phosphoglycerate kinase 2
Omim ID
172270Gene Ontology
HyperlinkGene Summary
The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. See also PGK1 (MIM 311800), which is ubiquitously expressed in all somatic tissues and maps to chromosome Xq13.[supplied by OMIM
Other Designations
OTTHUMP00000016591|phosphoglycerate kinase 1, pseudogene 2|phosphoglycerate kinase autosomal pseudogene
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Interactome
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Pathway
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from ornithine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carbon fixation in photosynthetic organisms
- Glycolysis / Gluconeogenesis
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Publication Reference
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Multiple elements influence transcriptional regulation from the human testis-specific PGK2 promoter in transgenic mice.
Zhang LP, Stroud J, Eddy CA, Walter CA, McCarrey JR.
Biology of Reproduction 1999 Jun; 60(6):1329.
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Analysis of the cDNA and encoded protein of the human testis-specific PGK-2 gene.
J R McCarrey, M Kumari, M J Aivaliotis, Z Wang, P Zhang, F Marshall, J L Vandeberg.
Developmental Genetics 1996 Jan; 19(4):321.
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A centromere-based genetic map of the short arm of human chromosome 6.
Blanche H, Zoghbi HY, Jabs EW, de Gouyon B, Zunec R, Dausset J, Cann HM.
Genomics 1991 Mar; 9(3):420.
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Multiple elements influence transcriptional regulation from the human testis-specific PGK2 promoter in transgenic mice.
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