LGR5 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of LGR5.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 451-480 at internal region of human LGR5.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Ammonium sulfate precipitation
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of LGR5 polyclonal antibody (Cat # PAB2591) in K-562 cell line lysates (35 ug/lane). LGR5 (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human brain tissue reacted with LGR5 polyclonal antibody (Cat # PAB2591), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. -
Gene Info — LGR5
Entrez GeneID
8549Protein Accession#
NP_003658;O75473Gene Name
LGR5
Gene Alias
FEX, GPR49, GPR67, GRP49, HG38, MGC117008
Gene Description
leucine-rich repeat-containing G protein-coupled receptor 5
Omim ID
606667Gene Ontology
HyperlinkOther Designations
G protein-coupled receptor 49|G protein-coupled receptor 67|orphan G protein-coupled receptor HG38
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Interactome
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Pathway
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Disease
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Publication Reference
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Metabolic improvement and liver regeneration by inhibiting CXXC5 function for non-alcoholic steatohepatitis treatment.
Seol Hwa Seo, Eunhwan Kim, Minguen Yoon, Soung-Hoon Lee, Byung-Hyun Park, Kang-Yell Choi.
Experimental & Molecular Medicine 2022 Sep; 54(9):1511.
Application:IF, Mouse, Mouse liver.
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Cytotoxic effect of disulfiram/copper on human cervical cancer cell lines and LGR5-positive cancer stem-like cells.
Hao-Zhe Cao, Wen-Ting Yang, Peng-Sheng Zheng.
BMC Cancer 2022 May; 22(1):521.
Application:WB-Tr, Human, HeLa, SiHa cells.
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LGR6 activates the Wnt/β-catenin signaling pathway and forms a β-catenin/TCF7L2/LGR6 feedback loop in LGR6 high cervical cancer stem cells.
Qian Feng, Shan Li, Hong-Mei Ma, Wen-Ting Yang, Peng-Sheng Zheng.
Oncogene 2021 Oct; 40(42):6103.
Application:IHC, WB-Ti, WB-Tr, Human, Mouse, Mouse model: Caski-xenograft tumors, HeLa-xenograft tumors, SiHa-xenograft tumors.
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Identification of stem cells in small intestine and colon by marker gene Lgr5.
Barker N, van Es JH, Kuipers J, Kujala P, van den Born M, Cozijnsen M, Haegebarth A, Korving J, Begthel H, Peters PJ, Clevers H.
Nature 2007 Oct; 449(7165):1003.
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Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors.
McClanahan T, Koseoglu S, Smith K, Grein J, Gustafson E, Black S, Kirschmeier P, Samatar AA.
Cancer Biology & Therapy 2006 Apr; 5(4):419.
Application:IHC-P, Human, Human colon cancer, ovarian cancer.
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Overexpression of orphan G-protein-coupled receptor, Gpr49, in human hepatocellular carcinomas with beta-catenin mutations.
Yamamoto Y, Sakamoto M, Fujii G, Tsuiji H, Kenetaka K, Asaka M, Hirohashi S.
Hepatology 2003 Mar; 37(3):528.
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Metabolic improvement and liver regeneration by inhibiting CXXC5 function for non-alcoholic steatohepatitis treatment.
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