PDPK1 (phospho S241) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PDPK1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S241 of human PDPK1.
Sequence
A-N-Sp-F-V
Host
Rabbit
Theoretical MW (kDa)
63
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Affinity chromatography
Concentration
1 mg/mL
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:1000)
Immunofluorescence (1:100-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from MDA-MB-435 cells using PDPK1 (phospho S241) polyclonal antibody (Cat # PAB25226).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDPK1 (phospho S241) polyclonal antibody (Cat # PAB25226).Immunofluorescence
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Gene Info — PDPK1
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Interactome
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Pathway
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Disease
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