ACSL4 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ACSL4.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 244 -274 at internal region of human ACSL4.
Sequence
IHSMQSVEELGSNPENLGIPPSRPTPSDMAI
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HepG2 whole cell lysate with ACSL4 polyclonal antibody (Cat # PAB2504) followed by HRP conjugated goat anti-rabbit IgG (H+L) as second antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the ACSL4 polyclonal antibody (Cat # PAB2504) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — ACSL4
Entrez GeneID
2182Protein Accession#
ACSL4_HUMANGene Name
ACSL4
Gene Alias
ACS4, FACL4, LACS4, MRX63, MRX68
Gene Description
acyl-CoA synthetase long-chain family member 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme preferentially utilizes arachidonate as substrate. The absence of this enzyme may contribute to the mental retardation or Alport syndrome. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000023846|acyl-CoA synthetase 4|fatty-acid-Coenzyme A ligase, long-chain 4|lignoceroyl-CoA synthase|long-chain fatty-acid-Coenzyme A ligase 4
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Interactomes
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Pathways
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Diseases
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Publication Reference
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An Evaluation of Fatty Acid-CoA Ligase 4 in Breast Cancer.
Tsai CH, Sheen-Chen SM, Huang CY, Chen YJ, Chi SY, Ko SF, Lee YW, Liang JL, Hsu HW, Huang EY, Chen HC, Huang CC, Rau KM, Tang RP.
Anticancer Research 2014 Mar; 34(3):1239.
Application:IHC, Human, Breast cancer tissue.
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ACSL3 and GSK-3β are essential for lipid upregulation induced by endoplasmic reticulum stress in liver cells.
Chang YS, Tsai CT, Huangfu CA, Huang WY, Lei HY, Lin CF, Su IJ, Chang WT, Wu PH, Chen YT, Hung JH, Young KC, Lai MD.
Journal of Cellular Biochemistry 2011 Mar; 112(3):881.
Application:WB-Ce, Human, HepG2, Huh-7 cells.
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An Evaluation of Fatty Acid-CoA Ligase 4 in Breast Cancer.
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